Confocal Fluorescence Imaging

Image Analysis

Training

Experimental Design

The confocal digital imaging unit is located in Room H1710 in the Health Sciences Centre. 
The unit operates on a fee-for-service basis, and is available to both internal and external users.

Hours of Operation

Monday through Friday
9:00am-5:00pm. (Fall & Winter)
9:00am-4:30pm (Summer)

Equipment and Service

Zeiss LSM 900 with Airyscan 2 Inverted biological microscope 4 lasers: -405 nm -488 nm -561 nm -640 nm Objectives: 5X, 20X, 40X, and 63X (oil). Many capabilities including: confocal optical sectioning, live-cell imaging, multi-channel imaging, time-lapse, Z-stack, tile scanning, FRAP, FLIP, definitive focus, and spectral imaging. User training is provided for a fee.
Olympus Fluoview FV1000 laser scanning microscope Inverted biological microscope 6 lasers with SIM scanner           - 405 nm LD           - 488 nm Argon           - 515 nm Multi Argon           - 543 nm Green HeNe           - 568 nm Krypton/Argon           - 633 nm Red HeNe Objectives: 10X, 20X, 40X, and 60X (oil). Broad application support including: multi-colour imaging, co-localization, and live-cell imaging. User training is provided for a fee.
Olympus Fluoview FV300 laser scanning microscope Upright biological microscope 3 lasers Equipped with a SC50 Olympus 5 megapixel digital colour camera.

Scheduling and Data Policies

• Booking time is through the scheduler located in Room H1710, or via email (see below).
• A requisition is required at time of use.
• Users are responsible for removing data files at time of acquisition

Biosafety Guidelines

For all biosafety guidelines refer to Memorial University Biosafety Policies and Procedures Manual.
All samples transported to the laboratory must be in a sealed container according to biosafety
guidelines. Laboratory coats and PPE must be worn at all times. 

Please Note: Users must add room H1710 to their current Biosafety certificate to use the facility.

Acknowledgements

Please remember to acknowledge any contributions of the EFCF in your publications, presentations, posters, and grant applications.

If the staff have provided technical and scientific advice beyond the routine, please consider them for authorship in your publications if appropriate.  Otherwise, a simple recognition in the acknowledgments sections such as the following is appreciated: “We thank Memorial University’s Medical Laboratories-Electron Microscopy/Flow Cytometry/ Confocal Microscopy for their support with this work.”

Educational Resources
 
Zeiss
Olympus
Leica
Nikon
Ibidi
Mattek

Spectra of Fluorochromes

FluoroFinder
BioLegend
ThermoFisher

Mailing Address:
Confocal Imaging Unit
Room H1710
Medical Laboratories
Faculty of Medicine
300 Prince Philip Drive
St. John’s, NL, A1B 3V6
Canada

Contact Persons: