The Genetic Code
The Central Dogma: DNA makes RNA
makes protein
In principle: The DNA genotype does not produce the phenotype directly
A DNA gene contains the information
necessary for the production of proteins,
which is expressed biochemically through an
intermediate molecule, RNA,
which functions as a Genetic Code
The Genetic Code is ...
a messenger RNA (mRNA) code
DNA carries genetic information,
but is not the 'Genetic Code' in the
biochemical sense
in 64 codons : 61 for amino acids + 3 'stops' [Table]
mRNA codons are read 5'3'
20 amino acids:
note 1- & 3-letter abbreviations
[more
on
amino
acids
&
proteins
in next section]
For example,
Degenerate: most amino acids are encoded by
more than one codon
first
two
positions
are
critical:
third position can "wobble" [see next section]
if third can be either puRine (R), or either pYrimidine
(Y)
two-fold degeneracy
if third can be any base
four-fold degeneracy
Leucine (leu) has six-fold degeneracy with
six codons in unusual arrangement:
Amino
Acid |
# codons |
trp,
met |
1 @ |
ser,
arg, leu |
6 @ |
ile |
3 @ |
Tyr, Cys,
His, Gln, Asn, Lys, Asp, Glu
|
2 @ |
Ser, Pro,
Arg, Thr, Val, Ala, Gly |
4 @ |
Unambiguous: any one triplet codes for only
one amino acid
but not vice versa, because of wobble
'Always' begins with an 'start' or 'initiator'
codon:
AUG
'Always' ends with a 'stop' or 'terminator' codon: UAG, UAA, or UGA
Universal (with some important exceptions)
Five Kingdoms
(animals, plants, algae, fungi, & monera)
use
same code for nuclear DNA
(nucDNA)
Organelles (chloroplasts
& mitochondria) have separate genomes:
cpDNA & mitochondrial DNA codes
evolutionarily modified
e.g., UGA codes for trp
(W) in vertebrate mtDNA
code
AUN may
act as Start Codons in invertebrate
mtDNA code
Stop codons may be
formed by addition of "A"s to transcript
Lab exercises use mtDNA data, so the mtDNA codes are
important
27+
alternative codes in various evolutionary lineages
Alteration & Variation in the
Genetic Code: Mutations & SNPs
Mutations
- interchanges of
one base type for another
transitions -
alternative pyrimidines [
CT ]
or purines [ AG ]
transversions - purines pyrimidines [C /
T A
/ G]
Recognized in
individuals & populations as SNPs ("snips": single
nucleotide polymorphisms)
[SNPs, Mutations, & Mutants:
a note on terminology & some lessons from
history]
Alternative nucleotide sequences
of a gene
correspond to alternative alleles
or: a single gene occurs
in
variant forms (alleles)
Single-base mutations
Consequences of exon
SNPs depend on position in triplet
3rd position
typically a silent mutation -
if position "wobbles", no change to amino acid
sometimes a mis-sense
mutation -
results in different amino acids
2nd position - always a mis-sense mutation
1st position - almost always a mis-sense
replacement
[Leu codons a major
exception]
stop codon mutations may occur at any position: coding non-coding
triplet
non-sense (termination) mutations terminate polypeptides
prematurely
: Identify all
codons one
step away from a termination codon
mutations in non-coding DNA have variable
effects
Ex.: mutations in promoter
regions
mutations at intron / exon
splice junctions
Insertion /
Deletion ("indel")
mutations
gain or loss of one or two nucleotides alters the reading
frame
frameshift mutations
(examples)
single & double nucleotide
indel downstream amino acids change
non-sense mutation eventually (quickly) produced
triplet indel - insertion /
deletion of single amino acid
typically milder consequences
multiple triplet insertions produce major effects
Ex.: CGG repeats in "Fragile X" Syndrome
length mutations - very large
indels (102~6 bps)
Genes
are highly
polymorphic (w/ multiple alleles) wrt their SNP variation
[Concept of "wild type" allele is
erroneous]
Text material ©2024 by Steven M. Carr