Allelic differences
due to single SNPs
ASOs used as
SNP-specific probes
"Southern Blot" test
without electrophoresis, probe "sticks" to only one allele:
Genetic screen
"positive or negative"
result
Ex.: 508-ASO Test for Cystic
Fibrosis probes deletion
mutation (OMIM219700)
Pre-Natal Screening:
the GATACCA
model
Ex.: Mr
& Ms Mus seek genetic counseling ....
Species-Specific Oligonucleotides (SSOs)
DNA Fingerprinting
Determination of an individual-specific
genetic patterns
Variable Number of Tandem
Repeat (VNTR) loci
Microsatellites
- di-, tri-, tetra-nucleotide motifs repeated 101s ~ 106s of times
(CACACACACACA
versus CACACACA)
Origin by slipped-mismatch
replication
Copy number
mutates rapidly, even between parents & children
VNTR loci are highly polymorphic
[HOMEWORK]
good marker for within-population
studies
[Animation of DNA
Fingerprinting for forensics
]
Forensics - use of DNA
data as legal evidence [CODIS]
Positive
identification of individuals [HOMEWORK]
with high probability [IG1 21.06]
CODIS
Multiplex
Fingerprinting
"Variant Detector Arrays (VDAs)" detect specific SNP alleles in genome
"GeneChips" perform automated "Northern"
analysis:
specific
mRNAs or cDNAs detected in proteome
"Heat Map" indicate levels of
gene expression by cool & hot colors
DNA "re-sequencing
(iterative) arrays" sequence ~500 Kbp in one experiment
All text material ©2024 by Steven M. Carr