Gel electrophoretic detection of RFLP variation

    SNPs (single-nucleotide polymorphisms) that create or destroy restriction sites lead to RFLPs (restriction fragment length polymorphisms) detectable by gel electrophoresis. The outside lanes are molecular size standards. Lanes 2 & 5 show the typical pattern of a single 8 Kbp fragment. Lanes 1 & 3 show two variants that result from the presence of either of two additional restriction sites. Lane 4 shows a variant of the genotype in Lane 3, with an extra restriction site.

    The restriction maps shown at right cannot be reconstructed from the gel data shown, but could be mapped with reference to data from other restriction enzymes.


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