Optical Microscopy and Flow Cytometry

Fluorescence Activated Cell Sorter (FACS)

Our florescence activated cell sorter (FACS) is a BD Special Order based on the Aria platform. It has a very versatile set-up and is capable of 4-way sorting of cells, viruses and other small organisms based on scatter and fluorophore detection gates.

 

  • Blue (488nm) and Red (633nm) lasers.
  • Four nozzle sizes (70, 85, 100 and 130 um).
  • Temperature controlled sort collection chamber (4 - 42°C) that can accomidate a variety of tubes, slides and well plates.

BD FACS Aria equipment manual

BD FACS DIVA software manual

Optical Microscopy Unit

Our microscopy unit is well equipped with a variety of Nikon microscopes, and features an advanced confocal system. This system is ideally suited for live cell imaging with a temperature and CO2 controlled incubation chamber and resonance scanning. 

For samples of images produced in the CDRF, see the  gallery.

Cold-Ocean Deep-Sea Research Facility (CDRF)

Ti-E confocal microscope

A1 confocal system with epifluorescence, motorized stage, incubation chamber, anti-vibration table, high-end objectives, perfect focus system, resonance scanning, software imaging and data analysis, 4 laser selections (405, 488, 561 and 640nm)
Cold-Ocean Deep-Sea Research Facility (CDRF)

Zeiss Axio Scan.Z1

Zeiss Axio Scan.Z1 brightfield and florescence slide scanner

Our slide scanner can autmatically digitize up to 100 slides in one run at 10x, 20x or 40x magnification. These files may be downloaded to use on you own computer with the free Zeiss Zen software or served online using the Zen browser for collaboration or educational purposes.

Click here for more information.

Cold-Ocean Deep-Sea Research Facility (CDRF)

Eclipse Ti-S inverted microscope

  • Bright field
  • Phase contrast
  • Fluorescence microscopy
  • Live cell imaging, digital camera
    • UV/Dapi
    • GFP
    • Texas Red
Cold-Ocean Deep-Sea Research Facility (CDRF)

SMZ745Y Steromicroscope

  • 0.67 to 5x zoom
  • Tabletop boom stand
  • 2 MP USB color digital camera

SMZ1500 Steromicroscope

  • 0.75 to 11.25x zoom
  • Transmited light stand with oblique coherent contrast
  • Camera port
Cold-Ocean Deep-Sea Research Facility (CDRF)

Eclipse Ci-L

  • Phase contrast, darkfield and simple polarizing microscopy

Eclipse TS100-F inverted microscope

  • LED illumination
  • Phase contrast