Agilent 1290 UHPLC-UV Sciex 7600 ZenoTOF (qTOF)

• Agilent 1290 UHPLC-UV
  • Binary pump with a flow range from 0.05 to 5 mL/min up to 1300 bar
  • Multisampler with sample drawers for well plates and individual sample containers (2 x 54 vial containers); injection volume from 1 – 20 μL or 100 μl
  • Thermostatted multicolumn compartment (20 °C - 110 °C)
  • 1260 DAD WR (Diode Array Detector) with wavelength range from 190 nm to 950 nm
  • UHPLC-UV can be used independently on a separate station with OpenLab software

• Sciex 7600 ZenoTOF (qTOF)
  • Turbolon Spray Source with Twin Sprayer ESI probe
  • Mass range for the TOF: up to 40 kDa. Precursor ion selection: m/z 5-2250
  • Mass resolution, positive ESI: equal to or greater than 42,000 (FWHM) measured on the [M+6H]⁶⁺charge isotope cluster for bovine insulin at 956 m/z
  • Mass accuracy over time, external: Less than 2ppm RMS over 12 hours of LC-MS
  • Sensitivity TOF MS – positive ESI: Reserpine 1pg on column for m/z 609.2806, with a 10mDa MEW (mass extraction window), S/N > 2500
  • Sensitivity Zeno MS/MS - positive ESI Reserpine 50 fg on column using the fragment m/z 195.0651, with a 10mDa MEW S/N > 400
  • Two novel technologies:
    • The Zeno trap that enables higher sensitivity
    • An EAD (electron activation dissociation) cell that enables an alternative fragmentation
  • Operating modes:
    • Full-scan TOF MS
    • Full scan TOF MS/MS
    • MRM HR
    • IDA
    • Zeno SWATH
  • SCIEX OS software for data acquisition and processing
  • Separate syringe pump (for infusion)
• Funding Canada Foundation for Innovation (CFI) via the Innovation Fund program and Government of Newfoundland & Labrador department of Industry, Energy & Technology through a grant led by Dr Chris Kozak and collaborators

Agilent 1290 UHPLC- 6495D Triple Quadrupole LC/MS/MS

• Agilent 1290 UHPLC-UV
  • Binary pump with a 0.05 - 5 mL/min flow range and up to 1300 bar
  • Vial sampler with two trays (2 x 66 vial containers); injection volume from 1 – 20 μL
  • Thermostatted multicolumn compartment  with a 4 °C - 110 °C temperature range
  • 1260 DAD WR (Diode Array Detector) with 190-950 nm wavelength range

• Agilent 6495D Triple Quadrupole LC/MS/MS
• Ion sources:
  • AJS ESI (jet stream technology)
  • APCI
• Mass range: 5-3000 m/z
• Resolution: unit= 0.7 Da
• Scan speed: 18,700 Da/s
• Sensitivity: reserpine IDL(instrument detection limit) < 0.4 fg
• Operating modes:
  • Scan
  • SIM
  • MRM
  • Product Ion
  • Precursor Ion
  • Neutral Loss
  • Neutral Gain
  • dMRM
  • tMRM and
  • mixed
• MRM speed: up to 700 MRM transitions
• Mass Hunter 12.0 for Data Acquisition, Qualitative and Quantitative processing

• Funding: Canada Foundation for Innovation (CFI) via the Innovation Fund program and Government of Newfoundland & Labrador department of Industry, Energy & Technology through a grant led by Dr Chris Kozak and collaborators

HPLC-MS (TOF): Agilent 1260 Infinity LC-6230 TOF LC/MS

• HPLC-UV
• Quaternary pump with a flow range from 0.05 to 5 mL/min against up to 600 bar
• Auto sampler that holds 100 samples, with injection volume of 1-100 µL
• Thermostatted column compartment
• 1260 DAD (Diode Array Detector) with wavelength range from 190nm to 640nm
• Reverse and normal phase chromatography possible
• Reverse phase HPLC columns available for use
• For sample preparation, please follow the guidelines available under Links

• MS with orthogonal TOF analyzer
• ESI (Electrospray) for large polar compounds. Mass correction available.
• APPI (Atmospheric Pressure Photoionization) for medium molecular weight, nonpolar compounds, that are not amenable by ESI.
• Mass range:
• High resolution and extended dynamic range 50 - 3,200 m/z
• Extended mass range: 100 - 20,000 m/z
• Resolution: 6,000 at 118 m/z and 15,000 at 2721 m/z
• Mass accuracy: 1-10 ppm

• Software: MassHunter Workstation version B.05.01
• Funding: Canada Foundation for Innovation (CFI) through Prof. Graham Bodwell

MALDI: Bruker ultrafleXtreme MALDI TOF TOF

• Mass range up to 500 kDa
• Linear Mode:
• mass resolution: ≥ 1,100 for Myoglobin (m/z 16,952) FWHM
• mass sensitivity: 500 fmol BSA (m/z 66,000) at S/N ≥ 100:1 shown with 1,000 laser shots
• mass accuracy

• Reflector Mode:
• mass resolution: ≥ 40,000 for Somatostatin 28 (m/z 3,147.47) FWHM
• mass resolution (protein mass range): ≥ 25,000 for Cytochrome C (m/z 12,361) FWHM
• MS sensitivity: 250 amol [Glu1]-Fibrinopeptide B (m/z 1,570.7) at S/N ≥ 200:1 shown on Bruker AnchorChip™ target with 2,000 laser shots
• mass accuracy (peptide mixture): ≤ 5 ppm with external calibration

• Operating modes: MS and Lift (MS/MS)
• FlexControl, FlexAnalysis, Polymerix and MestRe Nova software available for data processing.
• Acquired through a CFI by Professors Christopher Kozak and Christina Bottaro 

GC-MS / FID: Agilent 8890- 5977 MSD and FID

• Separation, quantitative and qualitative analysis of volatile and semi volatile compounds
• PAL auto sampler with 162 vial’ tray
• Liquid injection for routine semi volatile compounds’ analysis
• Static headspace and dynamic headspace (purge and trap) for the determination of volatile and semi-volatile analytes in liquids and solid matrices
• Multimode inlet injectors
• HP-5MS UI column installed (non-polar with low bleed characteristics); other columns available too
• GC is interfaced to a single quadrupole 5977 MSD, mass range of 1.6 - 1090 Da
• EI and CI ionization with methane reagent gas
• NIST library, 2020 edition
• FID detector
• Mass Hunter Data acquisition and Data Processing software
• For sample preparation, please follow the guidelines available under Links
• Funding Canada Foundation for Innovation (CFI) via the Innovation Fund program and Government of Newfoundland & Labrador department of Industry, Energy & Technology through a grant led by Dr Chris Kozak and collaborators

GC-MS: Agilent 7890A GC – 5975C MSD Inert XL EI/CI

• Separation, quantitative and qualitative analysis of semi volatile compounds 
• EI ionization and CI ionization with methane reagent gas
• Split/splitless injector
• Auto sampler with 100-vial tray
• Zebron ZB-5MS PLUS column (low polarity column with low bleed characteristics
• GC is interfaced to a single quadrupole 5975 inert MSD with a mass range of 1.6 - 1050 m/z
• Unit mass resolution over mass range
• For sample preparation, please follow the guidelines available under Links
• Funding through Prof. Cora Young