Detection of
Satellite DNA by density gradient ultracentrifugation
In an ultracentrifuge
experiment, the
bulk of nuclear DNA has
a characteristic [G+C]
content, which gives it a characteristic range of
density that corresponds to
the main band (MB)
in a cesium chloride
(CsCl) density gradient. The presence of repetitive DNA with
a
distinctive [G+C]
content was first detected by
smaller "satellite" bands with a
lower density.