Restriction map of White-Tailed Deer mitochondrial DNA
The mitochondrial DNA (mtDNA)
molecule of vertebrates is a circular molecule about 17,000 bp
(17 Kbp) in circumference. Double-digest
analysis of mtDNA from a White-Tailed Deer (Odocoileus
virginianus) of eastern North America identifies the
positions of 33 six-base-pair restriction sites from 10
different restriction endonucleases (letter codes a,
b, c, e, f, h, j,
x, y, & z). This gives a restriction
map of the molecule based on 198bp, about 1% of the
genome.
The restriction
map can be oriented to the functional map of gene regions in the
molecule by the placement of the two highly-conserved Sst
II sites [z] in the 12S
& 16s rRNA genes at an
interval of 1.6kbp. This map was part of one of the
earliest uses of variation among restriction maps to look at
relationships within and between closely related species,
including western Mule Deer (O. hemionus) [SM Carr
et al. 1986. Proc Natl Acad Aci 83:9576] .