
Restriction map of White-Tailed Deer mitochondrial DNA
    The mitochondrial DNA (mtDNA)
        molecule of vertebrates is a circular molecule about 17,000 bp
        (17 Kbp) in circumference. Double-digest
            analysis of mtDNA from a White-Tailed Deer (Odocoileus
          virginianus) of eastern North America identifies the
        positions of 33 six-base-pair restriction sites from 10
        different restriction endonucleases (letter codes a,
        b, c, e, f, h, j,
        x, y, & z). This gives a restriction
          map of the molecule based on 198bp, about 1% of the
        genome. 
      
    The restriction
        map can be oriented to the functional map of gene regions in the
        molecule by the placement of the two highly-conserved Sst
          II sites [z] in the 12S
          & 16s rRNA genes at an
        interval of 1.6kbp. This map was part of one of the
        earliest uses of variation among restriction maps to look at
        relationships within and between closely related species,
        including western Mule Deer (O. hemionus) [SM Carr
          et al. 1986. Proc Natl Acad Aci 83:9576] .