Beadle and Tatum
grew spores from each
of the Neurospora heterotroph
strains
on a series of minimal media, each supplemented with a
different amino
acid. Each of the heterotroph strains had a requirement for a specific
amino acid. They inferred that a separate mutation in each
strain affected synthesis of that amino acid. In this example,
supplementing the minimal
media with tyrosine permits growth of the heterotrophic
mutant: the inference is that a gene mutation has affected the
ability to synthesize tyrosine,
and this strain is called a tyr-
mutant.