The Hershey & Chase
Blendor Experiment (Part 2)
During the
process of infection of bacteria by phage, a quick spin
in a Waring blendor shears phage away from the outside
of the bacteria. Analysis of the bacterial pellet and supernatant
fractions demonstrates that 32P phosphorus-labelled DNA
inside the
phage shows up inside bacteria
(left), whereas the 35S
sulfur-labelled protein in the external coat remains in
the supernatant (right).
Further, phage labelled with 32P go on to
produce progeny phage that are also labelled. This biological
experiment confirms Avery's conclusion that DNA
is the transforming substance, and shows that
transformation is inherited.
HOMEWORK:
The Hershey & Chase blendor experiment is often described as
"quick & dirty": Why? Explain, in terms
of the key figure in Hershey & Chase (1952)
(below).