Genetic Engineering of a modified Ti plasmid

A small intermediate vector is constructed that contains the gene of interest, as well as two antibiotic resistance genes (spcR & kanR). The intermediate vector is genetically recombined with a "disarmed" form of Ti that lacks the tumor-gall production genes, but which does contain portions of the T-DNA region that are necessary for insertion into the plant chromosome.


Figure © 2000 by Griffiths et al. ; Text material © 2003 by Steven M. Carr