2,7-Diamino-10-ethyl-9-phenyl-phenanthridinium
bromide
Ethidium Bromide
Ethidium
bromide is an intercalating dye that was formerly
used as a nucleic acid stain in molecular biology. The
main three-ring portion resembles a flat purine-pyrimidine
base pair, and is able to slip between adjacent co-planar
DNA base pairs (intercalation).
Under
ultraviolet light, intercalated EtBr fluoresces with a strong pink-orange
colour, which makes very small amounts of DNA visible.
However, ethidium bromide is a very strong mutagen, carcinogen, and teratogen that causes
mutations, cancer, and developmental defects, respectively. It
has been replaced by other intercalating dyes, such as RedSafe.
All
text material ©2024 by Steven
M. Carr