Biology
4900/7931 - Research Methods in Genetic
Biotechnology
Dates: 26 April - 14 May 2010
Lecture Room:
SN-2101
Laboratory: SN-3119
Required: Bound lab
notebook,
lab coat, sensible shoes; glasses recommended
|
Overview The methods
of genetic biotechnology are becoming increasingly important to
researchers in diverse areas of biology. The current course will
provide an analytical and theoretical introduction to the methodologies
and a basis for Honours and graduate students to use and apply them in
their own research. This is a
three-week intensive hands-on course in DNA technology including
nucleic acid extraction and analysis, PCR, cloning and library
construction, sequencing, data analysis and bioinformatics, and project
design. To apply these techniques within a research framework, students
will investigate novel aspects of the molecular genetics and genomics
of endangered |
Instructors
Dr H. Dawn
Marshall (Biology
- Course Co-ordinator) dawnm@mun.ca
Dr Steve Carr
(Biology) scarr@mun.ca
Teaching
Assistant
Prerequisites
Biology 2250
(Principles of
Genetics) or Biochem equivalent
Biology 2060
(Principles of
Cell Biology)
Method
of Evaluation
|
|
Graduate |
Undergraduate |
|
Laboratory
Notebook |
45% |
50% |
|
Bioinformatics
Project |
25% |
30% |
|
Final Test |
20% |
20% |
|
Research
Proposal |
10% |
NA |
Reference
Materials
(All required
materials will
be provided -
Ø
Acquaah, G. 2004
Understanding
Biotechnology: An Integrated and Cyber-Based Approach
Pearson
- Prentice Hall
Ø
Mount,
D.
2004
Bioinformatics:
Sequence and Genome Analysis
Ø
Weissensteiner, T., H.G. Griffin, & A. Griffin, 2004
PCR
Technology:Current Innovations
CRC
Press
Ø
Gibson,
G.
and
Muse,
S. 2009
A
Primer of Genome Science, 3rd ed.
Sinauer
Associates
Ø
Sambrook, J. & D.W. Russell
Molecular
Cloning: A Laboratory Manual 3rd Edition
Ø
Seidman, L.A. & C.J. Moore, 2000
Basic
Laboratory Methods for Biotechnology
Prentice
Hall
Ø
Bloom,
M.
et
al.
1995
Laboratory
DNA Science
Benjamin
Cummings
Ø
Innis, M. 1989
PCR
Protocols: A Guide to Methods and Applications
Academic Press