Bio2250 - Sample
Restriction Mapping Problems
In each problem, three
restriction endonucleases are used to cut a piece of DNA, singly and in pairwise
combination. Sizes of fragments are listed in order of size, not in linear order.
Determine the correct order of restriction sites, and draw the
map, with the intervals between sites labelled.
Worked
Example
A) 11, 6, 5
B)
14,8
C)
16,6
A
x B) 8, 6, 5, 3
A
x C) 11, 5, 5, 1
B
x C) 8, 8, 6
D)
18,
5
E)
15,
7, 1
F)
20,
3
D x E) 10, 7, 5, 1
D x F) 15, 5, 3
E x F) 15, 4, 3, 1
G)
14,
1
H)
9, 6
I)
7, 5, 3
G x H) 9, 5, 1
G x I) 7, 5, 2, 1
H x I) 5, 4, 3, 3
Circular
Restriction Mapping
A)
17
B)
17
C)
10, 7
A
x B ) 12, 5
A
x C ) 10, 6, 1
B
x C ) 7, 6, 4
All
text
material ©2014 by Steven M. Carr