Spemann Experiment with the Grey Crescent (1902)
Hans
Spemann repeated the Roux / Driesch
experiments, using salamander eggs. Instead of killing
one cell or separating 2- or 4-cell embryos, Spemann devised a
micro-surgical technique that used extremely fine
baby's hair (from the head of his own newborn son!) to
separate the single-cell egg into two halves. The hair was
tied in a slip knot, passed around the egg as a ligature, and
gradually tightened without breaking the egg membrane
Spemann observed that the two halves often developed into two
complete embryos, consistent with the previous results of
Driesch. However, he also observed that this depended
critically on the plane of cleavage. If the division bisected
a well-known morphological feature of the egg cortex, the Grey
Crescent, both halves developed normally. If the
Crescent were confined to only one side of the cell, that half
would develop normally, but the other half would develop only
as a shapeless mass of undifferentiated cells. Spemann
reasoned that the Grey Crescent was a morphogenetic field
that determined the fate of subsequent cells.
Subsequently, it was determined that the
Grey Crescent is formed by the 30o rotation of the
fertilized egg to form a dorsal / ventral axis. The
crescent thus serves as a determinant of dorsal /
ventral polarity: cells without it have no means of
orientation, and fail to develop normally.
Similar experiments done with Xenopus frogs
in the FEB ("Frog Experiment Bay") on the Space
Shuttle confirmed this, where experiments in
micro-gravity also disrupted dorsal / ventral
orientation.
Text material
© 2022 by Steven M. Carr