Identifying the bone marrow cells affected by extracellular vesicles released from CD24-stimulated B cells

Rashid Jafardoustbostani
PhD Student
Department of Biochemistry

Date: February 26, 2024
Time: 1:00 p.m. to 2:00 p.m. 
Room: CSF 1302

Abstract:

Extracellular vesicles (EVs), which are small membrane-bound vesicles, serve as carriers of bioactive molecules such as DNA, RNA, proteins, and lipids. EVs play a crucial role in cell-to-cell communication. Our lab previously discovered that the engagement of the cell surface protein CD24 on B lymphocytes (B cells) causes the release of EVs. We found that stimulation of CD24 on the donor cells caused the transfer of lipids, CD24, and the B cell receptor (BCR) to recipient cells. Bone marrow is a crucial hematopoietic tissue responsible for producing and maturing various blood cells, including B cells. In the bone marrow, stromal cells play a critical role in regulating hematopoiesis, including B cell development. They provide important signals and interactions that influence B cells' proliferation, differentiation, and maturation. The objective of this study is to determine if EVs released by B cells can be taken up by bone marrow cells and the subsequent impact on B cell development. Our findings suggest that M2-10B4 bone marrow stromal cells are not the major target of the EVs released by CD24-stimulated B cells. Our future aim is to determine which cell types can uptake the EVs released by CD24-stimulated B cells in the bone marrow and the consequences of EV uptake on recipient cells. Overall, this study seeks to unravel the cellular interactions and functional consequences of EV uptake by bone marrow cells, focusing on the uptake of EVs released by CD24-stimulated B cells.