Prediction of Bioactive Peptides Produced From Camelina (Camelina Sativa (L.) Crantz) Meal Using In Silico Approaches

Prediction of Bioactive Peptides Produced From Camelina (Camelina Sativa (L.) Crantz) Meal Using In Silico Approaches

Na Thi Ty Ngo
PhD candidate
Department of Biochemistry
Memorial University

Monday, April 11, 2022
Time: 1-2 pm
Location: Arts and Administration Building Room 1046

Abstract

Camelina and sophia meals are valuable underutilised by-products of the oil-processing industry, which are high in protein at about 40%. Our previous studies revealed that their protein isolates and protein hydrolysates have desirable functional properties and antioxidant activities. Therefore, they can be considered a prime source of bioactive peptides to develop functional food ingredients and nutraceutical products. The predictions of bioactive peptides can be achieved by employing bioinformatics methods. The aim of this presentation is to focus only on utilizing camelina protein hydrolysates for releasing potent antioxidative and angiotensin-converting enzyme (ACE) inhibitory peptides, and dipeptidyl peptidase IV (DPP IV) inhibitor using various in silico approaches. The BIOPEP-UWM, ToxinPred, and AlgPred were used to analyse the peptide susceptibility to gastrointestinal enzyme degradation, toxicity, and allergenicity in silico. The results show the essential amino acid contents of camelina protein isolates and hydrolysates were comparable and satisfy the World Health Organization, and Food and Agriculture Organization (WHO/FAO) recommendations. We found that all digestive resistant peptides were nontoxic and displayed favorable functional properties, indicating that they may have the potential for being used in various food applications. In silico prediction of absorption, digestion, metabolism, and excretion profiles based on physicochemical properties and Lipinski's rule-of-five revealed that most peptides had desirable drug-like properties. This study provides insight into potential use of undervalued camelina processing discards for the development of functional food products or nutraceuticals.