Dr. Raymond Thomas - April 4, 2022
Application of Lipidomics to Better Understand Plant Acclimation to Environmental Stress in Boreal Ecosystem or Northern Climate
Application of Lipidomics to Better Understand Plant Acclimation to Environmental Stress in Boreal Ecosystem or Northern Climate
Raymond Thomas
Associate Professor, Boreal Ecosystems and Agriculture Sciences, School of Science and the Environment
Grenfell Campus, Memorial University
Monday, April 4, 2022
Time: 1-2 pm
Location: https://mun.webex.com/mun/j.php?MTID=m4a780b9b406f7c9faad16634461530a9
Abstract
Raymond Thomas, Sey, A., Pham, T., Cheema, M. Galagedara L., Nadeem, M.
Memorial University, Faculty of Science/Boreal Ecosystem and Agriculture Science, Grenfell Campus, Corner Brook, Newfoundland, Canada
Lipidomics is a relatively new branch of the omics family of techniques specifically concerned with the analysis of the lipidome in biological samples. Herein, we present a multimodal lipidomics approach to resolve modified, low abundance lipids and isomers in biological samples during routine analysis. We then demonstrate the application of the multimodal lipidomics approach to discover novel lipids in soil microbes and plants during their acclimation to environmental stressors in boreal or northern climates. Specifically, we show silage corn modulated their root membrane lipidome to produce elevated levels of PA and novel acylated-phosphatidylglycerol during acclimation to production on podzols in boreal climate. Canola remodeled their lipidome producing 2x the omega 3 fatty acids and monoacetyldiacylglycerides in response to cultivation at lower temperatures in boreal climate. High level of monomethylated phosphatidylethanolamine in the membrane of soil microbes present in the root rhizosphere of podzolic soil typical in boreal ecosystem. This work present novel findings of the application of lipidomics to better understand how the lipidome influence plant acclimation during cultivation on podzols in boreal climate or ecosystem.