Scoring
allozyme variation on electrophoretic gels:
Score each gel, using F M S for the 'fast', 'medium,' and
'slow' bands, starting with the top (fastest)
band as “F”. Gels 3 & 4 involve enzymes with a dimeric quaternary
structure, such that heterozygotes have a 3-banded pattern.
Gel 1 (Pgm –
Phosphoglucomutase)
(Example: lanes 5, 6, & 7 are FF, SS, and MS)
Gel 2 (Pgm –
Phosphoglucomutase)
Gel 3 (Pgi – Phosphoglucoisomerase)
note:
ignore the
top band in this gel
Gel 4 (6-Pgd – 6-Phosphogluconate
dehydrogenase)
From a lab exercise ©2005
by DJ Innes; text ©2008 by Steven
M. Carr